If your server is hosting ASP.NET 1.X and ASP.NET 2.0 applications then it is better to create the application pools and run the applications under designated application pools. Application pools allows you to separate the .NET 1.X and .NET 2.0 process from each other. You can create application pools in Window Server 2003 and IIS 6 by using the control panel -> IIS and click on the item which says "Application Pool". Once, you have created the application pool you can simply right click on the website folder, select properties and make it to run under the particular application pool.

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