You can create the SqlDataSource control in Asp.net 2.0 dynamically. Always remember to add the control to the Page control using the Controls.Add method. 
 

 protected void Button1_Click(object sender, EventArgs e)
    {
        SqlDataSource s = new SqlDataSource();
        s.ID = "mySqlSourceControl";
        Page.Controls.Add(s); 
                
        s.ConnectionString = ConfigurationManager.ConnectionStrings["MyDatabase"].ConnectionString;
        s.UpdateCommand = "UPDATE Person SET Name = @Name WHERE PersonID = 6";
        ControlParameter param = new ControlParameter();
        param.ControlID = "TextBox1";
        param.Name = "Name";
        param.PropertyName = "Text";
        s.UpdateParameters.Add(param);
        s.Update();
        
        s.DataBind(); 
        
           
    }